LymphedemaV1, Main, Exploration, bibRecord, 003696

EMILIN1/α9β1 Integrin Interaction Is Crucial in Lymphatic Valve Formation and Maintenance

Identifieur interne : 003696 ( Main/Exploration ); précédent : 003695; suivant : 003697

EMILIN1/α9β1 Integrin Interaction Is Crucial in Lymphatic Valve Formation and Maintenance

Auteurs : Carla Danussi ; Lisa Del Bel Belluz ; Eliana Pivetta ; Teresa Maria Elisa Modica ; Andres Muro ; Bruna Wassermann ; Roberto Doliana ; Patrizia Sabatelli ; Alfonso Colombatti ; Paola Spessotto

Source :

Molecular and Cellular Biology [ 0270-7306 ] ; 2013.

RBID : PMC:3838180

Descripteurs français

KwdFr :
- Animaux, Cartes d'interactions protéiques, Cellules cultivées, Cellules endothéliales (cytologie), Cellules endothéliales (métabolisme), Glycoprotéines membranaires (analyse), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Intégrines (analyse), Intégrines (métabolisme), Lymphangiogenèse, Mouvement cellulaire, Prolifération cellulaire, Souris, Souris de lignée C57BL, Souris knockout, Vaisseaux lymphatiques (malformations), Vaisseaux lymphatiques (métabolisme), Vaisseaux lymphatiques (ultrastructure).
MESH :
- analyse : Glycoprotéines membranaires, Intégrines.
- cytologie : Cellules endothéliales.
- génétique : Glycoprotéines membranaires.
- malformations : Vaisseaux lymphatiques.
- métabolisme : Cellules endothéliales, Glycoprotéines membranaires, Intégrines, Vaisseaux lymphatiques.
- ultrastructure : Animaux, Cartes d'interactions protéiques, Cellules cultivées, Lymphangiogenèse, Mouvement cellulaire, Prolifération cellulaire, Souris, Souris de lignée C57BL, Souris knockout, Vaisseaux lymphatiques.

English descriptors

KwdEn :
- Animals, Cell Movement, Cell Proliferation, Cells, Cultured, Endothelial Cells (cytology), Endothelial Cells (metabolism), Integrins (analysis), Integrins (metabolism), Lymphangiogenesis, Lymphatic Vessels (abnormalities), Lymphatic Vessels (metabolism), Lymphatic Vessels (ultrastructure), Membrane Glycoproteins (analysis), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Mice, Mice, Inbred C57BL, Mice, Knockout, Protein Interaction Maps.
MESH :
- chemical , analysis : Integrins, Membrane Glycoproteins.
- abnormalities : Lymphatic Vessels.
- cytology : Endothelial Cells.
- chemical , genetics : Membrane Glycoproteins.
- metabolism : Endothelial Cells, Integrins, Lymphatic Vessels, Membrane Glycoproteins.
- ultrastructure : Lymphatic Vessels.
- Animals, Cell Movement, Cell Proliferation, Cells, Cultured, Lymphangiogenesis, Mice, Mice, Inbred C57BL, Mice, Knockout, Protein Interaction Maps.

Abstract

Lymphatic vasculature plays a crucial role in the maintenance of tissue interstitial fluid balance. The role of functional collecting lymphatic vessels in lymph transport has been recently highlighted in pathologies leading to lymphedema, for which treatments are currently unavailable. Intraluminal valves are of paramount importance in this process. However, valve formation and maturation have not been entirely elucidated yet, in particular, the role played by the extracellular matrix (ECM). We hypothesized that EMILIN1, an ECM multidomain glycoprotein, regulates lymphatic valve formation and maintenance. Using a mouse knockout model, we show that in the absence of EMILIN1, mice exhibit defects in lymphatic valve structure and in lymph flow. By applying morphometric in vitro and in vivo functional assays, we conclude that this impaired phenotype depends on the lack of α9β1 integrin engagement, the specific lymphatic endothelial cell receptor for EMILIN1, and the ensuing derangement of cell proliferation and migration. Our data demonstrate a fundamental role for EMILIN1-integrin α9 interaction in lymphatic vasculature, especially in lymphatic valve formation and maintenance, and underline the importance of this ECM component in displaying a regulatory function in proliferation and acting as a “guiding” molecule in migration of lymphatic endothelial cells.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838180

DOI: 10.1128/MCB.00872-13
PubMed: 24019067
PubMed Central: 3838180

Affiliations:

Le document en format XML

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<term>Cellules endothéliales (métabolisme)</term>
<term>Glycoprotéines membranaires (analyse)</term>
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<term>Souris de lignée C57BL</term>
<term>Souris knockout</term>
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<term>Vaisseaux lymphatiques (métabolisme)</term>
<term>Vaisseaux lymphatiques (ultrastructure)</term>
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<term>Membrane Glycoproteins</term>
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<term>Lymphatic Vessels</term>
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<front><div type="abstract" xml:lang="en"><p>Lymphatic vasculature plays a crucial role in the maintenance of tissue interstitial fluid balance. The role of functional collecting lymphatic vessels in lymph transport has been recently highlighted in pathologies leading to lymphedema, for which treatments are currently unavailable. Intraluminal valves are of paramount importance in this process. However, valve formation and maturation have not been entirely elucidated yet, in particular, the role played by the extracellular matrix (ECM). We hypothesized that EMILIN1, an ECM multidomain glycoprotein, regulates lymphatic valve formation and maintenance. Using a mouse knockout model, we show that in the absence of EMILIN1, mice exhibit defects in lymphatic valve structure and in lymph flow. By applying morphometric <italic>in vitro</italic>
 and <italic>in vivo</italic>
 functional assays, we conclude that this impaired phenotype depends on the lack of α9β1 integrin engagement, the specific lymphatic endothelial cell receptor for EMILIN1, and the ensuing derangement of cell proliferation and migration. Our data demonstrate a fundamental role for EMILIN1-integrin α9 interaction in lymphatic vasculature, especially in lymphatic valve formation and maintenance, and underline the importance of this ECM component in displaying a regulatory function in proliferation and acting as a “guiding” molecule in migration of lymphatic endothelial cells.</p>
</div>
</front>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Colombatti, Alfonso" sort="Colombatti, Alfonso" uniqKey="Colombatti A" first="Alfonso" last="Colombatti">Alfonso Colombatti</name>
<name sortKey="Danussi, Carla" sort="Danussi, Carla" uniqKey="Danussi C" first="Carla" last="Danussi">Carla Danussi</name>
<name sortKey="Del Bel Belluz, Lisa" sort="Del Bel Belluz, Lisa" uniqKey="Del Bel Belluz L" first="Lisa" last="Del Bel Belluz">Lisa Del Bel Belluz</name>
<name sortKey="Doliana, Roberto" sort="Doliana, Roberto" uniqKey="Doliana R" first="Roberto" last="Doliana">Roberto Doliana</name>
<name sortKey="Modica, Teresa Maria Elisa" sort="Modica, Teresa Maria Elisa" uniqKey="Modica T" first="Teresa Maria Elisa" last="Modica">Teresa Maria Elisa Modica</name>
<name sortKey="Muro, Andres" sort="Muro, Andres" uniqKey="Muro A" first="Andres" last="Muro">Andres Muro</name>
<name sortKey="Pivetta, Eliana" sort="Pivetta, Eliana" uniqKey="Pivetta E" first="Eliana" last="Pivetta">Eliana Pivetta</name>
<name sortKey="Sabatelli, Patrizia" sort="Sabatelli, Patrizia" uniqKey="Sabatelli P" first="Patrizia" last="Sabatelli">Patrizia Sabatelli</name>
<name sortKey="Spessotto, Paola" sort="Spessotto, Paola" uniqKey="Spessotto P" first="Paola" last="Spessotto">Paola Spessotto</name>
<name sortKey="Wassermann, Bruna" sort="Wassermann, Bruna" uniqKey="Wassermann B" first="Bruna" last="Wassermann">Bruna Wassermann</name>
</noCountry>
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</record>

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Serveur d'exploration sur le lymphœdème

EMILIN1/α9β1 Integrin Interaction Is Crucial in Lymphatic Valve Formation and Maintenance

EMILIN1/α9β1 Integrin Interaction Is Crucial in Lymphatic Valve Formation and Maintenance

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
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